Gene description for TFG
Gene name trafficking from ER to golgi regulator
Gene symbol TFG
Other names/aliases HMSNP
SPG57
TF6
TRKT3
Species Homo sapiens
 Experiment description of studies that identified TFG in Embryonic kidney cells

1
Experiment ID1408
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36706192   
OrganismHomo sapiens
Experiment descriptionDisplaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
AuthorsChoi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.
Journal name Sci Adv
Publication year2023
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

2
Experiment ID1413
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36706192   
OrganismHomo sapiens
Experiment descriptionDisplaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
AuthorsChoi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.
Journal name Sci Adv
Publication year2023
SampleEmbryonic kidney cells
Sample nameHEK293T - Fraction 5
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density1.151 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

5
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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